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KMID : 1098420170250060361
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Korean Journal of Medicinal Crop Science
2017 Volume.25 No. 6 p.361 ~ p.366
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Determination of the Origin of Angelica Roots using Angelica gigas Chloroplast Based SSR Markers
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Park Sang-Ik
Hwangbo Kyeong
Gil Jin-Su
Chung Hee
Kim Ho-Bang
Kim Ok-Tae
Kim Seong-Cheol
Koo Sung-Cheol
Um Yu-Rry
Lee Yi
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Abstract
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Background: In the herbal medicinal industry, Angelica gigas Nakai, Angelica sinensis (Oliv.) Diels. and Angelica acutiloba (Siebold & Zucc.) Kitag. are often confused, because the roots of the three species can not be distinguished by their appearance. This confusion can cause serious side effects. In this study, we determined the origins of Angelica roots distributed in the Korean market using the simple sequence repeat (SSR) markers developed based on the A. gigas chloroplast DNA sequence.
Methods and Results: We collected twenty seven A. gigas and three A. acutiloba samples from the Seoul, Daegu, and Cheongju herbal medicinal markets. Fifty sections of one collection were mixed and ground to make a powder, which was used for DNA extraction using the cetyl trimethylammonium bromide (CTAB) method. Chloroplast based SSR markers were applied to the DNA for the determination of the species. In addition, polymorphism was found in eight samples. The phylogenetic analysis showed that the A. gigas roots collected from herbal medicinal markets were clearly discriminated from A. sinensis and A. acutiloba even though they were grouped into four clusters.
Conclusions: This study showed that chloroplast based SSR markers would help the discrimination of Angelica roots in the Korean herbal medicinal industry and the markers are useful to prevent confusion between Angelica roots.
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KEYWORD
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Angelica gigas Nakai, Chloroplast, Simple Sequence Repeat, Species Discrimination
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